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KMID : 0386320000330010107
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Korean Leprosy Bulletin
2000 Volume.33 No. 1 p.107 ~ p.114
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Identification of regulatory sequence within the promotor of M.leprae-specific 18-kDa gene and its binding factor(s)
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Kim Min-Joo
Chae Gue-Tae
Kim Yong-Kyu
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Abstract
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A central question to the pathogenesis of leprosy is how Mycobacterium leprae, the
carsative agent of leprosy, survives and replicates within macrophages. 18-kDa protein
of M. leprae, a major antigen, is found in solely M. leprae and contains T-cell antigenic
epitopes and has been implicated in survival of M. leprae within macrophages and
ultimately in pathogenesis. The latter is supported further by a recent finding that
18-kDa gene is activated during intracellular growth. To further understand M.
leprae-specific 18-kDa gene expression regulation mechanism during intracellular growth,
the present studies have been undertaken.
To examine the presence of a regulatory sequence(s) in the promoter of 18-kDa gene
and its binding factor(s) in M. laprae cell lysate. a gel mobility shift assay was
performed. A 350-6p sequence containing the promoter of 18-kDa gene resulted in a
protein-DNA complex formation with increasing amounts of M. leprae crude lysate.
However, the protein -DNA complex formation was not detected in the presence of a
nonspecific carrier, salmon sperm DNA.
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